Developing Biofuel Bioprocesses Using Systems and Synthetic by Sylvia M. Clay, Stephen S. Fong

By Sylvia M. Clay, Stephen S. Fong

Advances in technological and analytical equipment have fostered quick development of structures biology and artificial biology. There is still quick adjustments and discoveries in either fields with a small variety of contemporary peer-reviewed stories indicating the various relationships among platforms biology and artificial biology. This proposed SpringerBrief will hide middle techniques of platforms biology and artificial biology and illustrate the implementation of linked study methodologies for an built-in method of particularly deal with engineering microorganisms for biofuel production.​

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Poly­mer­ase cycling assem­bly (PCA) runs sim­i­lar to a PCR and uses oli­go­nu­cle­ o­tides that all have flank­ing regions that com­bine to leave sin­gle-stranded gaps that a DNA poly­mer­ase then fills in. The DNA strands can be up to 50 base pairs and should over­lap about 20 base pairs. Sim­il­ar to PCA is another method called Iso­ther­mal Assem­bly (Gib­son Assem­bly) where there is an over­lap of about 20–40 base pairs and multiple strands of DNA can be joined in one reac­tion. Unlike PCA, Gib­son is an iso­ther­mal assem­bly that occurs at 50 °C and runs for up to an hour mak­ing it one of the quick­est assem­bly meth­ods.

References Adav SS, Ravindran A, Chao LT, Tan L, Singh S, Sze SK (2011) Proteomic analysis of pH and strains dependent protein secretion of Trichoderma reesei. J Proteome Res 10(10):4579–4596. 1021/pr200416t Alper H, Moxley J, Nevoigt E, Fink GR, Stephanopoulos G (2006) Engineering yeast transcription machinery for improved ethanol tolerance and production. Science 314(5805):1565–1568. 1131969 Arkin A (2008) Setting the standard in synthetic biology. Nat Biotechnol 26(7):771–774. 1038/nbt0708-771 Atsumi S, Wu TY, Machado IM, Huang WC, Chen PY, Pellegrini M, Liao JC (2010) Evolution, genomic analysis, and reconstruction of isobutanol tolerance in Escherichia coli.

Research in sys­tems biol­ogy has pro­vided much of the nec­es­sary bio­log­i­cal knowl­ edge that is needed to under­stand a cel­lu­lar sys­tem enough to attempt whole-cell S. M. Clay and S. S. 1 Schematic of dis­cov­ery-based and design-based approaches to bio­log­i­cal engi­neer­ing design. In addi­tion, com­pu­ta­tional bio­log­ic­ al mod­els are being used to pro­spec­ tively eval­u­ate the effects of per­tur­ba­tions on cel­lu­lar func­tion. Syn­thetic biol­ogy pro­gress has pro­duced genetic engi­neer­ing meth­od­ol­o­gies that enable almost any pro­posed genetic design to be directly imple­mented.

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