By Sylvia M. Clay, Stephen S. Fong
Advances in technological and analytical equipment have fostered quick development of structures biology and artificial biology. There is still quick adjustments and discoveries in either fields with a small variety of contemporary peer-reviewed stories indicating the various relationships among platforms biology and artificial biology. This proposed SpringerBrief will hide middle techniques of platforms biology and artificial biology and illustrate the implementation of linked study methodologies for an built-in method of particularly deal with engineering microorganisms for biofuel production.
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Extra info for Developing Biofuel Bioprocesses Using Systems and Synthetic Biology
Polymerase cycling assembly (PCA) runs similar to a PCR and uses oligonucle otides that all have flanking regions that combine to leave single-stranded gaps that a DNA polymerase then fills in. The DNA strands can be up to 50 base pairs and should overlap about 20 base pairs. Similar to PCA is another method called Isothermal Assembly (Gibson Assembly) where there is an overlap of about 20–40 base pairs and multiple strands of DNA can be joined in one reaction. Unlike PCA, Gibson is an isothermal assembly that occurs at 50 °C and runs for up to an hour making it one of the quickest assembly methods.
References Adav SS, Ravindran A, Chao LT, Tan L, Singh S, Sze SK (2011) Proteomic analysis of pH and strains dependent protein secretion of Trichoderma reesei. J Proteome Res 10(10):4579–4596. 1021/pr200416t Alper H, Moxley J, Nevoigt E, Fink GR, Stephanopoulos G (2006) Engineering yeast transcription machinery for improved ethanol tolerance and production. Science 314(5805):1565–1568. 1131969 Arkin A (2008) Setting the standard in synthetic biology. Nat Biotechnol 26(7):771–774. 1038/nbt0708-771 Atsumi S, Wu TY, Machado IM, Huang WC, Chen PY, Pellegrini M, Liao JC (2010) Evolution, genomic analysis, and reconstruction of isobutanol tolerance in Escherichia coli.
Research in systems biology has provided much of the necessary biological knowl edge that is needed to understand a cellular system enough to attempt whole-cell S. M. Clay and S. S. 1 Schematic of discovery-based and design-based approaches to biological engineering design. In addition, computational biologic al models are being used to prospec tively evaluate the effects of perturbations on cellular function. Synthetic biology progress has produced genetic engineering methodologies that enable almost any proposed genetic design to be directly implemented.